The Microcarrier and Cell Separation system is a completely sterile, single-use filtration system. This bag facilitates a separation process using a streamlined, single-use filter/mesh system and a peristaltic pump. Simply pump the unfiltered fluid to the inlet port, through the filtration mesh, and out the second port. By customizing the bag size, mesh size, and pump settings the user is able to trap and break up cell clumps, or trap microcarriers as required, all with the same recovery performance as current methods.